SpectraMax M5, Analyst HT or Analyst GT. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The corrected absorbance is within 5% of the. SpectraMax M2 and SpectraMax M2e are controlled by an external computer running SoftMax Pro software which provides integrated instrument control, data display, andQualify the fluorescence performance of the SpectraMax . The substrate solution (100 μL/well) was subsequently employed for 5-min reaction and the absorbance at 370 nm was monitored on a SpectraMax M5 plate reader (Molecular Devices). SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). SpectraMax Quant dsDNA Assay Kits. SpectraMax DuoLuc Reporter Assay (SMP 6. . Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. . For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax Microplate Reader platform. Settings not required by a reader are indicated by ‘---’. The SpectraMax M5 Microplate Reader uses SoftMax® Pro. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. ATP standard curves read on SpectraMax microplate readers. Maximum signal with lowest background. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. When the user applies a set temperature of 37°C, the top is heated to 37. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. The IP-One assay can be used to characterize compounds that act on Gq-coupled receptors in either adherent or suspension cells. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Formula accessors for reporting o The accessor!sectionname will return the name of the SoftMax Pro section containing the. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The ratioof the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation of Delta F (%)As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. ® reader to the computer. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Lift up the lid over the chamber to insert or remove a cuvette. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. . Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. . 2. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Description 1. No accessories are needed and SoftMax® Pro. Refer to “Installing Detection Cartridges” in. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. . Access this by going to Help >. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. D 1. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. Wavelength Range. Dual monochromators provide the ability for. Call 516-939-0888 to check current. Customer Satisfaction is our number one goal All lab equipment in stock. 1. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. View the Molecular Devices website. Add to Cart. . EarlyTox Live Cell Assay (6. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Molecular Devices. SOFTmax PRO UserÕs Manual iii Contents Figures . M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. 3: The cuvette chamber. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. SpectraMax M5e Multi-Mode Microplate Reader. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence intensity, glow luminescence, fluorescence polarization and time-resolved fluorescence (TRF). Do NOT use the front panel Ref button in fluorescence mode cuvette reads. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. 190 - 1100 nm. 1. . 72 MB. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Our absorbance plate readers feature our PathCheck Sensor technology and. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Place the plate into a SpectraMax M5 e plate reader (Molecular Devices). Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. SpectraMax Microplate Readers. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. STAKMAX. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The time course of the cleavage reaction was monitored by loss of FRET (excitation, 450 nm; emission, 655 nm) with 15 reads/well and 4–5-s read intervals on a SpectraMax M5 plate reader (Molecular Devices). SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Typical LLDs were approximately 1–2 fg/well in the Analyst GT and 2–3 fg/well in the SpectraMax M5. 5. Microplate Reader Shelf. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. Plate chamber design of the SpectraMax category 1 microplate readers. MDS Analytical Technologies announced the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers. When the user applies a set temperature of 37°C, the top is heated to 37. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Figure 1. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Molecular Devices also offers a mass spectrometry-based solution for food safety testingMOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. 3. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. Description 1. Table of Contents. FlexStation® 3 Multi-Mode Microplate Reader. Turn on the microplate reader. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Qualify the fluorescence performance of the SpectraMax . We would like to show you a description here but the site won’t allow us. . SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 多功能酶标仪 FlexStation3 快速动力学操. Step 1: Open the SoftMax Pro IMAP FP protocol. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. . The Spectr. Spectranet customer portalMice were fasted overnight and orally administered FITC-dextran (600 mg/kg). Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e• SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Time-Resolved Fluorescence (TRF) Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing. Sale. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. UV/Vis absorbance and fluorescence spectra were measured on SpectraMax® M5 Multi- Mode Microplate Reader. Small, unbound molecules rotate rapidly in solution (top), while large,. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. These lab workhorses are used in drug discovery, research, bioassay validation, and. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. The dynamic range of the assay was approximately 5 orders of magnitude. The user manuals cover installation, troubleshooting, and validation of the SpectraMax M5 and its accessories. D 1 1. The dual monochromator optics allow the widest range of applications to be utilized for bior. View the Molecular Devices website. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 2. view more . . 6: bioNova científica, s. 0 (GraphPad Software, La Jolla, CA, USA) and SPSS version 23. Description 1. The drawing shows a standard microplate of 15 mm height covered with a lid. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Choose from a three- (M3), four- (M4), or SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity,. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 4. No filter change with dual monochromator optics. . individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). New drivers take 6 days to write, existing drivers take 2 days to convert to Windows 7/10 compatibility. This application note describes how to. With optimized reagents, option to operate the readers. +1 800-635-5577. 0 (IBM Corporation, Armonk, NY, USA). The M5 can read volumes down to 2uL with the uMax Low Volume Plate. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Description 1. Last lab had ours for years and never had a problem with it. . 5. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. The PathCheck sensor can extend the instrument linearity by a factor of three or more. ScanLater Western Blot Detection Cartridge. Setup recommendations for SpectraMax® M5e. SpectraMax® Microplate Readers and SoftMax® Pro Software. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. Also refer to this Multimode Reader Comparison Chart. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5, Analyst HT or Analyst GT. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Step 4. 7°C and the bottom to 36. Published Dec 16, 2020 | Updated Dec 16, 2020. Lift up the lid over the chamber to insert or remove a cuvette. 00 USD. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. 检测模式包括： • 紫外-可见光 光吸收 (Abs) • 荧光强度 (FI) • 化学发光 (Lum) • 时间分辨荧光 (TRF) • 荧光偏振 (FP) SpectraMax M5e 在 M5 基础上通过了 Cisbio HTRF 检测认证而增加了应用范围。. Absorbance (UV – VIS) Fluorescence; Glow luminescence; Time-resolved fluorescence; Fluorescent polarization; ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX calibrated. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. . INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M5 Multi-Mode Microplate Reader. The absorbance and photo-luminescence was determined on a SpectraMax M5 plate reader via quartz cuvette using 1 × 10 10 particles per ml or lower and extrapolated when an optical density of 1 was. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The following document is intended to demonstrate setup of this instrument. 6. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Exhibit with us {{>currencyLabel}} Back. • Tel. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. 2. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. For example, microplates containing different samp les can be read using the same orPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 26 5039228D Note. The plate reader can measure multiple readouts, including UV. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. It is available to all researchers. . In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 313 reference gain check fail. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. 5. 304 signal level saturation. In the M2, M5 and Plus. Molecular Devices SpectraMax M5 Multimode Microplate Reader. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created DateThe Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. 0 mL). The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Table 1. SpectraMax M3, M4, and M5/M5e readers. HTRF is a proprietary time-resolved fluorescence technology that overcomes The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images were obtained with SU8010 field. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. The day after, a final concentration of 1 μmol/L ARS1620, 20 nmol/L SMARTpool siRNA or the combination of the 2 was added in 5 replicates (wells). Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. 1. For example, microplates containing different samples can be read using theLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. Table of Contents. Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. The chamber contains. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Purchase Overlord with the required number of. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. Figure 2. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. The following document is intended to demonstrate setup of this instrument. 2. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96. . Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. We would like to show you a description here but the site won’t allow us. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. These all-inclusive assay kits are ready to use in assay validation or screening. Multimode Readers Comparison Chart. The M5 can read volumes down to 2uL with the. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 4. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. 1) SpectraMax Glo Steady Luc Reporter Assay (6. SpectraMax®L Luminometer User Guide 0112-0174 B 11 The SpectraMax L Luminescence Microplate Reader can accommodate SBS-standard 96 and 384-well microplates with dimensions of 128. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. In either case. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. ISM Equipment Classification (Group 1, Class A) This equipment is. 1. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. . The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. ORDER. Microplate Readers include the M3, M4,. Italic and boldface type are used for emphasis. EUROPE. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. . edu) to be added as a trained user in iLab. Step 1: Open the SoftMax Pro IMAP FP protocol. 306 plate air cal. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. There were 12 replicate wells per dilution in both 96-well and 384- well plates. The SpectraMax M5/M5e & FlexStation 3 instruments use SoftMaxPro® Data Acquisition & Analysis Software. D 1. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. Spectral Range. The M5 can read volumes down to 2uL with the uMax Low. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. . . Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 95. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesand emission wavelengths for SpectraMax M5 fluorescence microplate readers are as follows: excitation at 490 nm and emission at 525 nm, with 515-nm emission cutoff filter (note that these are different from the wavelengths recommended for filter-based readers in the Quant-iT PicoGreen product insert). 315 can't find zero order. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Five preferred detection modes. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. 1). Flexible for 6-well to 384-well microplates. acquired on a SpectraMax M5 (Molecular Devices). SoftMax Pro Software combines reader control and data analysis and provides sophisticated curve fitting and statistics for more in-depth bioassay analysis. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Monday to Friday 7am - 5pm Pacific Time. . HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Lmicroplate luminometer. Cavicide (Warehouse, Cat # 79300360) 6. Three kits covering a wide range of dsDNA concentrations. The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. Read More. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. 315 can't find zero order. 2. D 1. These robust readers have been placed in labs from. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. 00 USD. Optimized for fluorescence microplate readers. SoftMax Pro. 316 grating motor driver faulty. After 4 h, the fluorescence intensity of serum FITC-dextran was obtained at 480 nm excitation wavelength and 520 nm emission wavelength using a Spectramax M5 microplate reader (Molecular Devices). TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The KeySpan USA-19HS is often required with new Windows 7. . There were 12 replicate wells per dilution in both 96-well and 384- well plates. Some luminescence applications, such as gene reporter assays, may require a luminometer with photon counting detection for greater sensitivity, such as the SpectraMax M5, Analyst GT or Analyst HT, or LMax II. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidePlace the plate into a SpectraMax M5 e plate reader (Molecular Devices). 7°C (gradient heating design). Our absorbance plate readers feature our PathCheck Sensor. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. ” SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. 3. Wavelength optimization. SpectraMax M5 microplate reader. TR-FRET assay. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 1step assay with SpectraMax microplate readers. For example, microplates containing different samples can be read using the same orOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. The following document is intended to demonstrate setup of this. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 0 out of 5 $ 17,999 $ 16,995. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. 0 out of 5 $ 17,999 $ 16,995. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 190-1000 nm, tunable in 1 nm increments. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Samples were measured together with a corresponding reference solvent contained in a. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Download PDF versions of the SpectraMax M5 User Guides from this page. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeWe would like to show you a description here but the site won’t allow us. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax ® Pro Software. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. 40266 Email: drugdiscoverytech@lifetech. Five preferred modes for diverse bioresearch applications. The Spectramax can accommodate cuvettes or a variety of 6 to 96-well plate formats. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. . 1) EarlyTox NucView 488 Assay (6. Description 1. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 1. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionHere, we present FluoroBrite™ DMEM, a cell culture media designed specifically for live-cell imaging that, when excited at wavelengths commonly used for FITC/GFP (i. Download PDF versions of the SpectraMax M5 User Guides from this page. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader Why does "Path?" appears in all wells when PathCheck is selected? Cause: Water Constants. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. . 19. Wavelength optimization. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. SoftMax Pro software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 01) between the GFP-transfected and mock-transfected cells at an initial seeding. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). The plate reader can measure multiple readouts, including UV and visible absorbance. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Wavelength Range. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic Gq-coupled receptor. Find regional support. NFkB Translocation is Proteasome-Dependent The proteasome is a large protein complex that degrades proteins into short peptides in a ubiquitin-dependent manner. Molecular Devices ®(MD) SpectraMax 340PC reader MD VersaMax™ Tunable Microplate readers MD SpectraMax® L Microplate Luminometers MD SpectraMax® M5 systems MD ®SpectraMax 96/384-well plate reader Gemini fluorescence plate reader Automation Hamilton Microlab® STAR™ liquid handling systemThe SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev.